http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110133145-A

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assignee http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f2a04d862a0a9d08f641f29e7547dbaf
classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02
filingDate 2019-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_df9ac9eec446cec15c6033e7a3bed70b
publicationDate 2019-08-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-110133145-A
titleOfInvention Liquid-phase chromatography method that is a kind of while detecting coreopsis tinctoria pigment synthesis precursor
abstract The invention discloses a kind of liquid-phase chromatography methods for detecting coreopsis tinctoria pigment synthesis precursor simultaneously, the following steps are included: reference substance stock solution is respectively prepared in catechin, epicatechin, dihydromyricetin and myricetin, different multiples are successively diluted again, establish the reference substance solution of various concentration gradient;The reference substance stock solution is drawn respectively or the reference substance solution is mixed to get mixed reference substance solution;Using high performance liquid chromatograph, to reference substance solution, sample introduction is analyzed, records peak area, and using reference substance content as abscissa, peak area is ordinate, draws standard curve;Liquid-phase chromatographic analysis is carried out to mixed reference substance solution again.The present invention can detect simultaneously proanthocyanidin synthesis precursor catechin, epicatechin, dihydromyricetin and myricetin under single wavelength, have the characteristics that operating method is simple, detection speed is fast, high sensitivity, favorable reproducibility.
priorityDate 2019-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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