http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110132946-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-3278 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-76 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-76 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-327 |
| filingDate | 2019-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110132946-B |
| titleOfInvention | Aptamer sensor and preparation method and application thereof |
| abstract | The invention discloses an aptamer sensor and a preparation method and application thereof, and the aptamer sensor is prepared from UiO-66-NH 2 Embedding signal molecule tris (2, 2' -bipyridyl) ruthenium dichloride by using metal organic framework material as loading matrix to obtain UiO-66-NH 2 /Ru(bpy) 3 2+ As a light emitter, the surface amino group of the light emitter is bonded to SDNA1 modified with a carboxyl group. SDNA1 is combined with thrombin aptamer through base complementary pairing, so that bioconjugate apt 1-gold nanoparticles are fixed on the surface of an electrode, and the plasma resonance effect of the gold nanoparticles can enhance UO-66-NH 2 /Ru(bpy) 3 2+ The ECL strength of the aptamer sensor is improved, and the sensitivity of the aptamer sensor is improved. When the target thrombin is added, the thrombin is specifically combined with the aptamer DNA apt1, so that Au NPs-apt1 gradually falls off from the surface of the electrode, the enhancement effect of the gold nanoparticles is weakened, and the aim of detecting the thrombin is fulfilled. The method can avoid the interference of background signals, and has stable optical signals and high sensitivity. |
| priorityDate | 2019-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 673.