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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64
filingDate 2019-05-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2021-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2021-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-110132921-B
titleOfInvention Method for monitoring enzyme digestion reaction in real time based on needle tip nanopore monomolecular detection technology
abstract The invention relates to a method for monitoring enzyme digestion reaction in real time based on a needle point nanopore monomolecular detection technology, which belongs to the technical field of fluorescence detection. The physical separation of the needle-point nano-hole to the detection area and the electrophoresis control of the movement of the molecule to be detected avoid the interference of other external factors to the reaction, the combination of the patch clamp and the fluorescence microscope technology can observe the reaction dynamic state in real time, and provide a more direct proof for the research of the enzyme digestion protection mechanism by the nano-material.
priorityDate 2019-05-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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