| abstract |
The present disclosure provides variant Pol6 polymerase polypeptides, compositions comprising Pol6 variant polypeptides, and methods of assaying nucleic acid sequencing (eg, by nanopore sequencing) using the variant Pol6 polypeptides. Variant Pol6 polymerases have reduced template-to-polymerase-template complex dissociation rates, which result in increased processivity relative to the parental Pol6 polypeptides from which they are derived. |