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http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-701
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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70
filingDate 2019-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2020-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2020-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-110093455-B
titleOfInvention Respiratory virus detection method
abstract The invention provides a high-throughput detection method for efficiently and quickly enriching respiratory viruses. The high-throughput detection and identification technology for efficiently and rapidly enriching the respiratory viruses comprises the following four parts: enrichment, library establishment, high-throughput sequencing and biological information analysis of respiratory tract sample viruses. The virus enrichment of respiratory tract samples is the core part of the technology, firstly, the nucleic acid of a host except the virus in the samples is removed by a biophysical method, and then the virus nucleic acid of the samples is extracted. The detection of an Agilent2100 instrument only uses 200pg of nucleic acid for library construction, and the results of high-energy sequencing and bioinformatics analysis show that the reads number of the virus is increased to more than 84% from less than 0.1% in the conventional method treatment, and the detection technology not only can quickly detect the infection of a sample with the virus, but also can improve the coverage rate (more than 95-100%) of the whole genome sequence of the virus, and greatly reduce the sequencing cost.
priorityDate 2019-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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