http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110093390-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate | 2019-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110093390-B |
| titleOfInvention | DNA guide-guided RecJ protein with endonuclease activity and application thereof in gene editing |
| abstract | The invention provides a DNA guide-guided BaRecJ protein with endonuclease activity and application of a mutant BaRecJM thereof in gene editing. The invention provides a RecJ nuclease from bacteria, which can combine phosphorylated small DNA, play the role of endonuclease on a specific site of complementary target DNA and edit genes; the amino acid sequence of the BaRecJ nuclease is SEQ ID NO. 1. In another aspect, the present invention also provides a mutant of the above-mentioned BaRecJ nuclease, thereby effectively weakening the activity of excision enzyme and enhancing the site-directed cleavage capability of target DNA guided by DNA guide; the amino acid sequence of the mutant BaRecJM of the RecJ nuclease is SEQ ID NO. 3. The Ago protein guided by DNA fragment for shearing reported at present is from thermophilic bacteria, and needs more than 65 ℃ to play the role. The BaRecJ and the mutant BaRecJM thereof can shear a DNA double strand under the mild condition of 37 ℃, thereby being more convenient for genetic engineering operation. |
| priorityDate | 2018-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 361.