http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110093315-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2502-1121 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2302 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-24 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0636 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0783 |
filingDate | 2019-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-110093315-B |
titleOfInvention | Peripheral blood memory T cell culture method |
abstract | The invention relates to a peripheral blood memory T cell culture method, which comprises the following steps: a) magnetic bead sorting of PBMC obtained by peripheral blood separation to obtain CD8 + A T cell; mixing the CD8 + Inoculating the T cells into a culture container coated with the CD3 antibody and the CD28 antibody for culture; the culture system contains autologous plasma, and the cell factors mainly comprise IFN-gamma and IL-2; b) changing the culture medium for amplification culture, wherein the new culture system contains autologous plasma, and the cytokines mainly comprise IL-2, IL-1a, IL-7 and IL-15; c) co-culturing the expanded T cells and DC cells; wherein the DC cells are pre-co-cultured with autologous tumor antigens. The invention can effectively expand the memory T cells in vitro and effectively improve the proportion of the active memory T cells. |
priorityDate | 2019-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 122.