http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110093297-B

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classificationCPCAdditional http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-01
classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-205
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y305-01041
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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-80
filingDate 2019-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2022-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2022-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-110093297-B
titleOfInvention Nitrate-reducing bacteria MCDA3-3 and its method for producing chitin deacetylase
abstract The invention discloses a seawater nitrate reducing bacteria MCDA3-3, CGMCC NO.16934. The strain is a Gram-negative short bacillus without spores. Grow on 2216E medium at 25°C for 3 days, the colony is round, taupe and translucent, with moist surface, regular edges, no halo, central protrusion, diameter 0.1‑0.5mm, easy to pick. Produces a yellow transparent circle on selection media containing p-Nitro-N-acetanilide and powdered chitin. The invention also discloses a method for producing chitin deacetylase by using the bacterial strain MCDA3-3, and the optimum fermentation conditions are: 4% inoculum size, 30°C, 180rpm fermentation for 48h. The optimum temperature of chitin deacetylase produced by MCDA3‑3 is 30°C, and the optimum pH is 8.0. Metal ions such as Sr 2+ can promote the activity of chitin deacetylase.
priorityDate 2019-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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