http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110078829-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K47-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K17-02 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K17-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K47-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-089 |
| filingDate | 2019-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110078829-B |
| titleOfInvention | Histidine-tagged protein fixed-point covalent immobilization method based on vinyl sulfone surface reaction |
| abstract | The invention discloses a novel method for fixed-point covalent immobilization of histidine-tagged protein based on vinyl sulfone surface reaction. The method for fixed-point covalent immobilization of the histidine-tagged protein takes a substrate material with a vinyl sulfone group on the surface as a protein immobilization carrier, and can realize the reaction of the histidine tag and the surface vinyl sulfone at room temperature in a protein solution under a near-neutral condition, thereby realizing the fixed-point covalent immobilization of the histidine-tagged protein on the surface of the vinyl sulfone. The novel fixed-point covalent fixation method has the advantages of strong directionality, high specificity, high stability of the immobilized protein, mild reaction conditions and simple experimental method, and the reagents used in the method are all conventional reagents and have low cost. The new method for covalent immobilization of histidine-tagged protein has wide application prospects in enzyme immobilization, biochip preparation, microarray preparation and targeted drug delivery system construction. |
| priorityDate | 2019-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 79.