abstract |
The present invention provides an African swine fever CD2V protein that can be highly expressed in CHO cell lines, and its amino acid sequence is SEQ ID NO: 4; the recombinant plasmid constructed by the present invention is used to express African swine fever virus CD2V protein in CHO cells The present invention also provides a recombinant CHO cell strain, which is prepared by transfecting CHO cells with the above-mentioned recombinant plasmid, which can be used to prepare CD2V protein, and the prepared protein can be used for differential diagnosis of African swine fever. The cell strain expressing the African swine fever CD2V protein of the present invention has high expression level, is easy to purify, can be used for differential diagnosis, and lays a solid foundation for the production of African swine fever subunit vaccines and diagnostic reagents. |