http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110068645-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-8813 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-88 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-88 |
| filingDate | 2019-04-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-09-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-09-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110068645-B |
| titleOfInvention | A method for the detection of cyclic adenosine monophosphate in urine by liquid chromatography-mass spectrometry |
| abstract | The invention discloses a method for detecting cyclic adenosine monophosphate in urine by liquid chromatography-mass spectrometry, comprising the following steps: (1) sample pretreatment: preprocessing the urine sample by anion exchange solid phase extraction to obtain a test solution; (2) Detecting the test solution by liquid chromatography-mass spectrometry: chromatographic conditions adopt reverse-phase C18 hydrophilic chromatographic column; gradient elution is adopted; mass spectrometry conditions are: ion source is electrospray ion source; ion mode is positive ion mode ; The monitoring mode is multiple reaction monitoring. The invention also discloses an internal standard quantitative detection method for detecting cyclic adenosine monophosphate in urine by liquid chromatography-mass spectrometry. The method of the invention is the first to establish the cAMP liquid chromatography tandem mass spectrometry detection method in the urine sample, which can effectively detect the cAMP in the urine sample, and has the advantages of good separation effect, stable baseline, short detection time, good linearity and high accuracy. Good reproducibility, high sensitivity and high precision. |
| priorityDate | 2019-04-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 218.