| abstract |
The present invention provides a high-yielding poly-γ-glutamic acid Bacillus and a construction method and application. The Bacillus is obtained by replacing the poly-γ-glutamate synthase promoter of Bacillus licheniformis with a replacement promoter. Specifically, the Bacillus licheniformis poly-γ-glutamate synthesis gene cluster pgsBCAE operon was replaced by a constitutive strong promoter P43, a stationary phase promoter Pylb, a tetracycline-inducible promoter Ptet, and a subtilisin synthase cluster promoter Psrf, respectively. promoter, four pgsBCAE promoter-replacement strains were obtained. The results showed that the poly-γ-glutamic acid production of the four engineered strains was significantly higher than that of the wild strains. The present invention adopts molecular biology technology to prepare an engineering strain capable of high-producing poly-γ-glutamic acid by replacing the poly-γ-glutamic acid synthase promoter of Bacillus with a replacement promoter, which is a high-producing poly-γ of Bacillus ‑Glutamate and other biological macromolecules provide a new strategy. |