http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110055305-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6804 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5308 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6804 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 |
| filingDate | 2019-04-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110055305-B |
| titleOfInvention | Method for detecting (CAG) n repeated sequence by using RNase H |
| abstract | The invention discloses a method for detecting (CAG) n repetitive sequences by using RNase H, belonging to the technical field of molecular biology. The method of the invention is carried out by mixing (CAG) n with a complementary RNA aptamer labeled with fluorescein; restriction reaction of restriction endonuclease RNase H: (CAG) n and aptamer are mixed evenly with restriction endonuclease RNase H solution and incubated in the mixed solution; fluorescence detection: adding graphene oxide into the solution subjected to the enzyme digestion reaction, uniformly mixing, incubating to obtain a solution to be detected, and detecting the fluorescence intensity by using a fluorescence spectrophotometer. The method has simple operation, rapid detection and high sensitivity, and the detection limit of (CAG) n is up to 1.12 multiplied by 10 ‑10 mol/L, the DNA sequence Containing (CAG) n detected by the invention has very high specificity. |
| priorityDate | 2019-04-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.