| abstract |
The invention provides an application of an esterase in splitting (R, S)-5-caprolactone, the amino acid sequence of the esterase is shown in SEQ ID NO.1, and the encoding gene thereof is shown in SEQ ID NO. 2 shown. The esterase gene of the present invention can be connected with an expression vector to construct an intracellular expression recombinant plasmid containing the gene, and then transformed into an Escherichia coli strain to obtain a recombinant Escherichia coli engineered strain; the recombinant Escherichia coli is cell broken, separated and purified to obtain a recombinant ester Enzyme; the recombinant esterase has the ability to catalyze the resolution of (R,S)-5-caprolactone, and can obtain (S)-5-caprolactone, the enantiomeric excess value>98% and the conversion rate reaching 49.6% , the splitting efficiency and product yield are high. |