Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d9de52bba13e16028dffa8775e3f3f28 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-4702 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 |
filingDate |
2019-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9c213677d61e3589350803d01e4fa58a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ac2fdbc92a478b854fe206c3f062cf96 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6038ff11d54a24a3097bd7e4db025eee http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cd2868dacaf522ef2ba17a883c96b733 |
publicationDate |
2019-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CN-110055254-A |
titleOfInvention |
The method of targeting knockout chicken IRF7 gene and its application in vaccine preparation |
abstract |
The invention discloses a kind of method of targeting knockout chicken IRF7 gene and its applications in vaccine preparation;Based on CRISPR/Cas9, design is directed to the gRNA target sequence of chicken IRF7 gene and is connected to VK001-02 plasmid, obtains chicken IRF7 gene targeting carrier;Targeting vector is transfected into chicken fibroblasts using liposome.After transfecting successful cell by puromycin screening, is screened through limited coubling dilution and obtain the chicken fibroblasts for knocking out chIRF7 gene.The knockout cell line shows that it can be such that virus titer increases through TCID50 verifying.This method effect of important transcription factor IRF7 and research of function suitable for the interferon expression access that chicken innate immunity pattern recognition receptors are mediated.The interferon beta expression that knockout cell line constructed by the present invention can be such that many congenital immunity accesses are mediated simultaneously is lowered, and is conducive to virus breeding, be can be used for amplification viral in vaccine preparation. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110684736-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111304172-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114107303-A |
priorityDate |
2019-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |