| abstract |
The invention discloses a promoter PCHF45 specifically expressed in rice pollen and its application, and relates to the fields of genetic engineering and molecular biology. The promoter PCHF45 of the present invention has the nucleotide sequence shown in SEQ ID NO. 1, and the present invention further provides an expression vector, gene expression cassette, engineering bacteria or cell line containing the promoter PCHF45. The present invention also provides primer pairs for amplifying pollen-specific promoters. The pollen-specific promoter of the invention is an endogenous gene of rice, which is very beneficial to rice genetic engineering, can drive the specific expression of exogenous genes in pollen with accurate expression level, and provides a method for driving the specific expression of exogenous genes in rice pollen. new method. |