http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110055209-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-04 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-04 |
| filingDate | 2019-03-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110055209-B |
| titleOfInvention | Preparation method of Talaromyces protoplast |
| abstract | The invention discloses a preparation method of a Talalae protoplast, belonging to the field of plant cell engineering. The preparation method comprises the following steps: the method comprises the following steps: s1: taking young leaves of the tylosin, cleaning the young leaves with sterile seawater, and temporarily culturing to obtain a raw material 1; s2: soaking the raw material 1 obtained in the step S1 in a pretreatment solution to obtain a raw material 2; s3: taking out the raw material 2 obtained in the step S2, sucking water, cutting into blocks, adding enzyme solution for enzymolysis to obtain an enzymolysis tissue; s4: filtering the enzymolysis tissue obtained in the step S3 to obtain filtrate; s5: centrifuging the filtrate obtained in the step S4, discarding the supernatant, suspending and precipitating with the heavy suspension solution, centrifuging, and discarding the supernatant to obtain a precipitate; s6: and suspending the precipitate obtained in the step S5 by using a heavy suspension solution to obtain the telangium protoplast. The invention establishes the preparation method of the tylosin protoplast for the first time, and the tylosin protoplast obtained by the method has high yield and high survival rate. |
| priorityDate | 2019-03-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 119.