| abstract |
Provided herein are methods for the efficient in vitro maintenance, expansion, culture and/or differentiation of pluripotent cells with disruption of the MeCP2 gene into various erythroid, myeloid, lymphoid or endodermal lineages, particularly mature erythrocytes. Pluripotent cells can be maintained and differentiated under defined conditions; thus, in certain embodiments, the use of mouse feeder cells or serum is not required for differentiating precursor cells. |