http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110042118-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0281b1316e5765ae59515f8dca1b6b6f |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-28 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P3-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P3-10 |
| filingDate | 2018-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ee520f22c0c3bae026ae1d13c18e8d0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c3a85fca48472fa4223e9d08603a3d58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f31945c6c8221f411bd24e59e1e86336 |
| publicationDate | 2019-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110042118-A |
| titleOfInvention | The viable bacteria biological capsule of rhodotorula glutinis transmits the application of insulin in vivo |
| abstract | The present invention relates to the application that a kind of viable bacteria biological capsule of rhodotorula glutinis transmits insulin in vivo, homologous recombination method constructs external source cytidine phosphate choline transferase gene CCT integrating expression vector, and it is intracellular that polypeptide is transferred to GM4-CCT using electroporation technology.The present invention realizes the high expression in rhodotorula glutinis GM4 bacterial strain of external source CCT gene, improves the lecithin content in recombinant bacterial strain GM4-CCT more than 1.5 times compared with wild strain；Allogenic polypeptide is successfully transferred to intracellular, and we verify the feasibility of the method with insulin, and it is the 1/2 of primary quantity that discovery insulin, which enters content intracellular, and polypeptide success is by package Entering fat drop；Mouse oral carries bacterial strain GM4-CCT-insulin continuous 7 days of insulin, it was found that the content of insulin increases in mice serum and vitals (liver, lung, spleen, kidney), reach highest when 2h after oral administration, wherein liver concentration reaches 5.36, by constructing diabetic mice animal model, Mouse oral recombinant bacterial strain GM4-CCT-insulin, compared with standard insulin, GM4-CCT-insulin has the function of significant hypoglycemic as exogenous insulin. |
| priorityDate | 2018-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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