| abstract |
The invention discloses a CpG-ODN with a specific immune stimulating effect on pigs and an application thereof. The nucleotide sequence of the CpG-ODN is shown in SEQ ID NO: 1. The CpG‑ODN can improve the induction of innate immunity and specific humoral and cell-mediated immune responses in pigs. It can be transferred into the plasmid vector pFAR4 to construct a recombinant plasmid pFAR4‑CpG, and the recombinant plasmid pFAR4‑CpG containing the CpG‑ODN can be prepared preparation. The preparation can not only effectively activate the natural immune function of pigs, as an immune activator, it can successfully replace or partially replace antibiotics; it can also effectively activate the acquired immune function of pigs, especially cellular immunity, and can be used as an immune adjuvant to improve vaccines. It has small toxic and side effects on the body, and has great potential, and can be widely used in the pig breeding industry, which is conducive to reducing breeding costs, reducing the incidence of pigs, and improving its survival rate and breeding efficiency. |