| abstract |
The present invention relates to the BHK-21 cell strain for stablizing expression MAP3K8 albumen and its buildings and application.The cell strain is named as BHK-21/MAP3K8, and deposit number is CCTCC NO:C2018261.Cell strain preparation: target gene MAP3K8 synthesis, recombined lentivirus vector Lv-MAP3K8 building, the slow virus of packaging expression MAP3K8, slow-virus infection BHK-21 cell obtain the BHK-21 cell strain for stablizing expression MAP3K8 albumen through screening, culture and identification.It compares with BHK-21, BHK-21/MAP3K8 cell strain is more advantageous to FMDV or SVV duplication, is more suitable for the development and production of FMDV or SVV vaccine, while providing reliable material to study the gene function of MAP3K8. |