| abstract |
The present invention relates to a 3,5-bis(2,4-dimethoxyphenyl)pyridine dicyclic ring, a preparation method and application thereof, and the compound structural formula is: . The novel macrocyclic nucleic acid dye of the present invention has yellow-green fluorescence, fast response, good stability, low cytotoxicity and high sensitivity, and can be used as a fluorescent dye for nucleic acid labeling. It can label double-stranded DNA and single-stranded DNA, and the fluorescence intensity difference of labeled single-stranded DNA at 529nm and 626nm is less than 10%, while the fluorescence intensity of labeled double-stranded DNA at 529nm and 626nm is quite different. to distinguish double-stranded and single-stranded DNA. |