| abstract |
The invention discloses a method for preparing chitosan-graphene oxide microspheres by spraying, comprising the following steps: (1) adding graphene oxide into an aqueous acetic acid solution, and ultrasonically dispersing it to form oxidized particles with a mass concentration of 0.5-10%. Graphene dispersion; (2) adding chitosan to graphene oxide dispersion, stirring and dissolving to form chitosan/graphene oxide dispersion; (3) spraying chitosan/graphene oxide dispersion The microspheres are prepared by drying technology; (4) collecting the microspheres, soaking the microspheres in a genipin solution for cross-linking, washing and drying to obtain the result. The preparation method of the invention is simple and efficient, and can realize continuous mass preparation of chitosan-graphene oxide microspheres; the prepared chitosan microspheres have uniform and controllable particle size, good dispersibility and good biocompatibility It can be used for the three-dimensional culture of stem cells. |