| abstract |
The invention relates to the field of species identification, in particular to a high-resolution melting curve-based identification kit and an identification method for Culex tritaeniae, characterized in that specific primers are designed according to the COIII gene sequence of Culex tritaeni , using the optimized high-resolution melting curve reaction system to identify various forms of Culex tritaeni, such as adults, eggs, larvae and pupae. It can identify Culex three-banded mosquitoes, with simple operation, fast speed, no post-processing of PCR products, detection of the difference of single bases, and real closed-tube operation. |