http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110029111-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c8dff647efafe2d6b7b5d7e2f9080211 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 |
filingDate | 2019-01-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e6609b06fb76e4a09dcc7ad6e40f6054 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2650ca01fde31abec4c252b0d36484a9 |
publicationDate | 2019-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-110029111-A |
titleOfInvention | Pichia pastoris produces the single-stranded method of recombination human source typeⅡ Collagen |
abstract | The invention discloses a kind of methods that Pichia anomala expression preparation and reorganization source of people typeⅡ Collagen is single-stranded.The present invention provides the nucleotide sequences that a kind of encoding recombinant human source typeⅡ Collagen is single-stranded, the nucleotide sequence is as shown in SEQ ID NO.1, and thus the single-stranded amino acid sequence of the encoded recombination human source typeⅡ Collagen of nucleotide sequence SEQ ID NO.1 is as shown in SEQ ID NO.2.The single-stranded full length amino acid sequence comprising mature 1 chain of human II type collagen α of recombination human source typeⅡ Collagen obtained according to the present invention, the realization of typeⅡ Collagen biological function can effectively be supported, with good biological activity, and aminoterminal, c-terminus contain different affinity purification labels, it can effectively purify and obtain overall length single chain protein, be also beneficial to the identification of collagen;The present invention carries out systematic calculation optimization, it is made to be more suitable for expressing in Pichia pastoris according to Pichia anomala expression feature, the gene order single-stranded to encoding recombinant human source typeⅡ Collagen simultaneously. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023098523-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111087478-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113045638-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022237224-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11787852-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113045638-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114276435-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111087478-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113185612-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20220155565-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113061179-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-102559311-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113185604-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113185604-B |
priorityDate | 2019-01-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 260.