http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110029064-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M33-04 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-26 |
| filingDate | 2019-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110029064-B |
| titleOfInvention | Method for separating and purifying water body microorganism bacteria and sampling device |
| abstract | The invention relates to a method for separating and purifying water body microorganisms and a sampling device, which comprises the following steps of 1, adding a sample to be separated and purified and a calcium carbonate solution into a sodium alginate solution to obtain a precipitate and a suspension; step 2, adding the suspension into an oil phase A consisting of sorbitan fatty acid ester and liquid paraffin to obtain a mixed system A; step 3, adding 6-10 ml of glacial acetic acid into the mixed system A, reacting for 8-12 min at 22-25 ℃ to obtain a mixed system B, adding a calcium chloride solution into the mixed system B to obtain microcapsules, and cleaning the microcapsules with sterilized water to obtain a microcapsule strain library; step 4, inoculating the microcapsule strain libraries into a porous plate one by one to culture to obtain a single bacterial colony, wherein the porous plate is filled with BM liquid culture medium solution of strains to be separated and purified; the invention combines the emulsification micro-balloon method and the porous plate method, and can be used for the establishment and separation of the environmental functional microbial ecological germplasm resources. |
| priorityDate | 2019-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 32.