http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110024689-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_70fbe08b7c540157200696115221f654 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-701 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 |
| filingDate | 2019-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_845d228dc4045ba7a79e3a985fe4c132 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3ff9ab543521501e19844f1790aa3149 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_40f5190d5197f7468b582984403ded51 |
| publicationDate | 2019-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110024689-A |
| titleOfInvention | Ultra-low temperature removal method and application of grape leaf roll virus-3 |
| abstract | The embodiments of the present specification provide a method and application for ultra-low temperature removal of grape leafroll virus-3. The method comprises the following steps: taking a single stem segment of a grape branch, sterilizing and sterilizing, substituting and culturing on a primary medium, substituting the stem segment of axillary bud germination on a proliferation medium for cultivation, and taking the shoot tip containing 5-6 leaf primordiums. Planted on pre-medium, cultured in the dark; pre-medium-cultivated shoot tips were treated in loading solution, then placed on ice, and then made into droplets; cryoprotectant-coated shoot tips were placed in liquid nitrogen , then transferred to the unloading solution, thawed and unloaded; the thawed shoot tips were transplanted to recovery medium for dark cultivation to obtain plants without grape leaf roll virus-3. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113907006-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113907006-B |
| priorityDate | 2019-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 47.