| abstract |
Provided herein is the efficient in vitro differentiation of HLA homozygous blood cell-derived pluripotent stem cells into hematopoietic precursor cells, and further differentiation of hematopoietic precursor cells into HLA homozygous immune cells of various myeloid or lymphoid lineages, particularly T cells , NK cells and dendritic cells. Pluripotent cells can be maintained and differentiated under defined conditions; thus, in certain embodiments, the use of mouse feeder cells or serum is not required for differentiation of hematopoietic precursor cells. |