http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110004211-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 |
filingDate | 2019-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-110004211-B |
titleOfInvention | Oligonucleotide set, nucleic acid sequence amplification method and target protein expression method |
abstract | The invention belongs to the technical field of biology, and discloses an oligonucleotide set, an amplification method of a nucleic acid sequence and an expression method of a target protein. Compared with the existing DNA gene detection, the invention has the following main advantages: (1) the method can be applied to places with poor conditions and insufficient resources, does not need special equipment, basically keeps the constant temperature of 37 ℃ in the formation of the secondary structure and the extension of the sequence, does not need specific temperature change requirements, and has wide application; (2) the operation of the experimental process is fast and convenient, only virus nucleic acid DNA and a detection Probe are needed to be added in the whole experimental operation process, and the Probe sequence can be designed in NUPACK within one minute to verify whether the designed sequence can obtain a required structure; (3) the sensitivity of the detection is more accurate than other experiments. The Trigger can be recycled, and can be accurately and quickly detected under the condition of detecting trace DNA nucleic acid. |
priorityDate | 2019-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 193.