| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fce53176770f3fb3034600e1e08634fc |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-31
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-32
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-25
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-125
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-155
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-44 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0609 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-075 |
| filingDate |
2019-04-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_02370f4297c5ae42dcc49d5276c72fdb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e92a4b351e6354a2df376cbe103e4e79
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_076acb1278cbeb430b0bff0cbd701916
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c4bea570138a56ab35b82cb9d9c56027
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9c60853dc6bd624ec41efa3093c71774
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e00a995fdd5db0ef5463aea0aff778d8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8c60a8c4a47b88154db2cceb9c2eb295
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3ffc977c719c1ba9f12f77bdaf2e8a75
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0a2b8ef8e0e3ae2ccc8da69bbbb295cf
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_202de71b3dad553b0ce11644b3af8038
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fa18a345c314a19da286004844c94709 |
| publicationDate |
2019-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-110004112-A |
| titleOfInvention |
A method for inducing human pluripotent stem cells to differentiate into oocytes arrested in meiosis II in vitro |
| abstract |
The invention discloses a method for inducing human pluripotent stem cells to differentiate into oocytes stagnated in meiosis II in vitro. The method is divided into three steps. First, the pluripotent stem cells with a cell density of 80% are replaced with PGC-m culture medium at 35-40°C and 3%-6% CO 2 for 10 days to obtain primordial germ cell-like cells; then transfer the formed primordial germ cell-like cells to PF-m medium, and at 35-40 Cultivated at 8% to 12% CO 2 for 5 days to obtain follicle-like structures; finally, the formed follicle-like structures were transferred to OLC-m medium and incubated for 10 to 15 days to obtain eggs that stopped at stage II of meiosis blast-like cells. The method of the present invention has high efficiency and short time, and the induced oocyte-like cells can exclude the first polar body and form secondary oocytes. This method provides a new means to study the formation and oogenesis of human female germ cells. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115197901-A |
| priorityDate |
2019-04-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |