| abstract |
The present invention provides a kind of recombination human source collagen and its application, the amino acid sequence of the recombination human source collagen is encoded as shown in SEQ ID NO:1, the recombination human source collagen successively includes from aminoterminal: aminoterminal affinity purification label, 2 chain maturation peptide chain of source of people I-type collagen α and c-terminus affinity purification label.The recombination human source collagen according to an embodiment of the present invention, there is specific affinity purification label by all designing at the both ends of 2 chain maturation peptide chain of source of people I-type collagen α, the recombination human source collagen is easily isolated purifying and detection, and the recombination human source collagen has higher molecular weight and special physicochemical property, it is easy to ferment and express, yield is high, and purposes is wide. |