http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109991337-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-047 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-045 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-062 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 |
| filingDate | 2019-04-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109991337-B |
| titleOfInvention | Method for simultaneously detecting four drugs and metabolites thereof in crab cream of Eriocheir sinensis |
| abstract | The embodiment of the invention discloses a method for simultaneously detecting four types of medicines and metabolites thereof in Eriocheir sinensis crab cream, which comprises the following steps: processing and homogenizing crab spawn to form pulpy crab spawn, adding mixed solution of acetonitrile and ethyl acetate, anhydrous magnesium sulfate and sodium chloride into the pulpy crab spawn, uniformly mixing, performing ultrasonic treatment, and centrifuging to obtain supernatant; purifying the supernatant through dispersive solid phase extraction to obtain a purified liquid, drying the purified liquid, dissolving and filtering a dried substance by using acetonitrile to obtain a liquid to be detected; and determining the four types of medicine compounds in the liquid to be detected and the content thereof by using liquid chromatography-mass spectrometry. The embodiment of the invention adopts the same pretreatment method, and obtains the optimal mixing ratio of acetonitrile and ethyl acetate through optimization, so that four types of medicines and metabolites thereof can obtain good measuring results. The extraction efficiency of the drugs in the actual positive sample is effectively improved, the working efficiency is greatly improved, the detection time is shortened, and the consumption of reagent consumables is saved. |
| priorityDate | 2019-04-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 128.