| abstract |
The invention discloses a human pluripotent stem cell culture medium and a preparation method and application thereof, wherein the cell culture medium contains DMEM medium, F12 medium, sodium selenate, sodium bicarbonate, vitamin C, insulin growth factor, Inhibitors of bFGF, transferrin and transforming growth factor beta signaling pathways. It is realized that it does not contain animal-derived components, and can provide sufficient nutrients and stable living environment for the cultured cells. At the same time, the preparation method has simple steps, easy to obtain raw materials, and the cultured cells have many generations, strong proliferation ability and good state. . |