http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109988238-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-622 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-2818 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2017-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109988238-B |
| titleOfInvention | Preparation method of PD1 single-chain antibody in Escherichia coli |
| abstract | The invention provides a preparation method of novel PD1 single chain antibody in Escherichia coli. The amino acid sequence of the PD1 single chain antibody fragment is shown in SEQ ID NO.1. The preparation method includes: cloning the gene of the amino acid sequence of the PD1 single-chain antibody with a signal peptide at the N-terminal into an expression vector with a promoter to construct a recombinant expression vector; transposing the obtained recombinant expression vector into competent cells, Screen the recombinant plaques, extract the recombinant plasmids, and transfect them into E. coli BL21 (DE3) competent cells to obtain recombinant bacteria for further screening, amplify and culture the bacteria to express PD1 single-chain antibody protein, and centrifuge to collect PD1-containing single-chain antibody Protein bacterial puree; after crushing the obtained bacterial puree, separate and purify to obtain PD1 single-chain antibody fragments. |
| priorityDate | 2017-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 149.