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publicationDate 2019-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-109971779-A
titleOfInvention A kind of construction method of new new gold mycobacteria expression system
abstract The present invention relates to new golden mycobacterium technical fields, and disclose a kind of new new gold mycobacteria expression system, it is made of cloning vector transfecting host, cloning vector is terminated with base sequence in promoter 3 ', the cloning vector from 6 ' to 4 ' successively constructs the cloning site of cloning vector, including following operability element: 286 skeleton plasmid of Ye Pulaci, Exogenous source expression casette, Screening markers expression casette;286 plasmid of Ye Pulaci is yeast episomal plasmid, target gene is connected with after SD sequence, Exogenous source expression casette successively contains ribose DNA promoter, IRES nucleic acid sequence, Exogenous source gene expression frame, rDNA terminator from top to lower section;Screening markers expression casette includes promoter, Screening markers gene, terminator, and cloning vector is inserted into cloning site.The construction method of the new new gold mycobacteria expression system has the advantages of capable of expressing under no inductive condition new golden mycobacterium.
priorityDate 2018-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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