http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109971779-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_869c35a50e04e9bc232d0f48cf1ff29f |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 |
filingDate | 2018-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7a3a4e53efddfea10a5f4ac6d3a18cdd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ec6eba46649a5240d48575dc6950a7f4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_212210cf52fd1e7b1a5b70caf302e802 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b1e1efb2ad5a75da42a50f3759b2cacf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fa2b602a4fd56b2271ab94888468daf0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a88bcc864cd28a5e426756c863e82bca http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_19820b01c838a6fe44ab36949a66376a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_019686725aecabaf946f138a259379ba http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a13bc01823c3a5ab764d677e87e43861 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6015de29c703a25ac164bf7b957e9818 |
publicationDate | 2019-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-109971779-A |
titleOfInvention | A kind of construction method of new new gold mycobacteria expression system |
abstract | The present invention relates to new golden mycobacterium technical fields, and disclose a kind of new new gold mycobacteria expression system, it is made of cloning vector transfecting host, cloning vector is terminated with base sequence in promoter 3 ', the cloning vector from 6 ' to 4 ' successively constructs the cloning site of cloning vector, including following operability element: 286 skeleton plasmid of Ye Pulaci, Exogenous source expression casette, Screening markers expression casette;286 plasmid of Ye Pulaci is yeast episomal plasmid, target gene is connected with after SD sequence, Exogenous source expression casette successively contains ribose DNA promoter, IRES nucleic acid sequence, Exogenous source gene expression frame, rDNA terminator from top to lower section;Screening markers expression casette includes promoter, Screening markers gene, terminator, and cloning vector is inserted into cloning site.The construction method of the new new gold mycobacteria expression system has the advantages of capable of expressing under no inductive condition new golden mycobacterium. |
priorityDate | 2018-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 43.