http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109971778-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-10 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-74 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 |
filingDate | 2017-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-109971778-B |
titleOfInvention | A combination of vectors for rapid gene editing in Halomonas and its application |
abstract | The invention provides a carrier combination for rapid gene editing in Halomonas and its application. The vector combination includes a cas9 expression vector and an sgRNA expression vector, wherein the cas9 expression vector is obtained by introducing the cas9 gene into a plasmid vector, wherein the cas9 expression vector does not contain the λ-RED recombinase gene. The present invention is faster than the existing gene editing methods of Halomonas, and only 8 days are needed for a gene editing process, which greatly improves the transformation efficiency of Halomonas. In addition, the present invention uses the combination of the above-mentioned vectors in Halomonas, and uses the CRISPR/Cas9-based gene editing method to perform continuous editing (knockout and insertion) of multiple genes, which can greatly reduce the time required. |
priorityDate | 2017-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 632.