| abstract |
The invention provides a luciferase fluorescence complementary system, a preparation method and application thereof, the system comprises Rab1, SidM/DrrA, luciferase C-terminal, luciferase N-terminal, connecting peptide and reducing agent TCEP, the The C-terminus of Rab1 is linked to the N-terminus of luciferase through a linking peptide, and the SidM/DrrA is linked to the C-terminal fragment of luciferase through a linking peptide; the present invention introduces specific interacting protein pairs and designs suitable flexible peptides and binding positions, optimize the type and concentration of the reducing agent in the buffer, and finally significantly improve the fluorescence complementation efficiency, up to 30 times; the preparation method provided by the invention is simple and efficient, easy to operate, easy to popularize, and has broad application prospects and market value . |