http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109957555-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1051 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 |
| filingDate | 2017-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109957555-B |
| titleOfInvention | Glycosyl transferase mutant and application thereof in catalyzing biosynthesis of gastrodin |
| abstract | The invention provides a glycosyltransferase mutant and application thereof in catalyzing biosynthesis of gastrodin, belonging to the technical field of genetic engineering. The glycosyltransferase mutant has an amino acid sequence shown as SEQ ID No.1 in a sequence table. The invention takes a mutant UGT73B6MK of glycosyltransferase UGT73B6 in rhodiola sachalinensis as a parent, and mutates the site of phenylalanine Phe (Phe) (F) at position 389 into alanine Ala (A), thereby improving the enzyme activity for catalyzing and synthesizing gastrodin. The in vitro enzyme activity of the modified glycosyltransferase mutant is parent UGT73B6 MK 4.9 times of that of the parent UGT73B6, and the in vivo conversion rate is higher than that of the parent UGT73B6 MK 3.8 times of the amount of the gastrodin, has better catalytic activity and is more suitable for the industrial production of gastrodin. |
| priorityDate | 2017-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 291.