http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109957015-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_03abaf060221c7eefd4e2c0b69b14295 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-76 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2039-505 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K9-0019 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-395 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P1-16 |
| filingDate | 2019-04-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e6a4844f188c0c0efebd3b39013b1c2a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_23f2f1971e4044094105aabdb0c364b5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d364139bbdd8969cf9ebad3c10038b1a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0fa51182d8fe0239cd6bb890d81b1fc5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_68b39705ad10f77232dc44cecd8e25d9 |
| publicationDate | 2019-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109957015-A |
| titleOfInvention | A kind of preparation method and application of HMGB1 blocking antibody |
| abstract | The invention relates to a preparation method of HMGB1 blocking antibody, which comprises the following steps: overexpressing the immunogen in Escherichia coli, and purifying by Ni-affinity chromatography; immunizing BALB/C mice with the immunogen, selecting the most Mice with optimal immune response were fused; mouse splenocytes were fused with SP2/0 myeloma cells to generate hybrid cells, clones were cultured, and stable clones were selected; hybrid cells were injected into the peritoneal cavity of mice, and 10 Ascites after ‑14 days, carry out antibody screening and testing, use protein A/G affinity chromatography or antigen affinity chromatography to purify ascites fluid, obtain HMGB1 blocking antibody, and verify the blocking effect of the antibody. The present invention also relates to the HMGB1 blocking antibody prepared by the above preparation method and its application. The present invention can effectively relieve liver inflammation caused by non-alcoholic fatty liver by blocking the effect of HMGB1 by neutralizing antibody, and provide theoretical and experimental evidence for clinical application of HMGB1 neutralizing antibody in treating non-alcoholic fatty liver in the future. |
| priorityDate | 2019-04-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 335.