http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109943577-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-02 |
| filingDate | 2018-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109943577-B |
| titleOfInvention | A kind of biotransformation method of anti-AIDS drug atazanavir intermediate |
| abstract | The invention discloses a biotransformation method of an anti-AIDS drug atazanavir intermediate, belonging to the field of medical biotechnology. The secondary structure and codon preference of a gene are adjusted, and the primer length is controlled within 60 bases to obtain primers. The obtained primers were dissolved in double distilled water, added to the reaction system, the prepared PCR reaction system was amplified, the obtained DNA fragments were purified by cutting gel, and cloned into the NdeI of pET30a by the method of homologous recombination. /XhoI site, the successfully sequenced DNA sequence is SEQ ID NO.3, named Sst-2, and its corresponding amino acid sequence is SEQ ID NO.4. The invention has mild reaction conditions, low equipment requirements, no high temperature or cooling in the production process, low energy consumption, convenient purification, single enzyme catalysis for the entire system, high coenzyme cycle times, and mild reaction conditions. |
| priorityDate | 2018-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 612.