http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109943546-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-07 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 |
| filingDate | 2019-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109943546-B |
| titleOfInvention | Glutamine transaminase mutant and preparation method and application thereof |
| abstract | The invention belongs to the technical field of bioengineering, and particularly relates to glutamine transaminase and a preparation method and application thereof. The invention extracts Bacillus subtilis ATCC 23857 genome DNA, obtains a wild glutamine transaminase btg gene sequence with an zymogen region by PCR amplification, and randomly mutates the wild btg gene obtained by amplification by error-prone PCR to obtain a mutant gene btgm. Constructing a recombinant vector by the mutant gene, successfully expressing the recombinant vector in bacillus subtilis, bacillus amyloliquefaciens and bacillus licheniformis to obtain a recombinant strain with improved enzyme production activity, and further optimizing by a fermentation process to obtain the novel glutamine transaminase. |
| priorityDate | 2019-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 209.