| abstract |
The invention discloses a recombinant Escherichia coli for accumulating glucosamine and an application thereof, belonging to the technical field of genetic engineering. Using Escherichia coli as the host to express, by knocking out four genes in the glucosamine synthesis pathway in Escherichia coli: acetylglucosamine transporter encoding gene nagE, mannose phosphate transporter encoding gene manXYZ, deacetylase gene nagA and deamination The enzyme gene nagB gene was used to obtain recombinant Escherichia coli, and the recombinant Escherichia coli was applied to the production of glucosamine, realizing the blocking of the transport pathway of the product glucosamine from extracellular to intracellular; and by overexpressing the gene encoding glucosamine synthase , The gene encoding glucosamine acetylase strengthens the synthetic pathway of glucosamine, thereby increasing the production of glucosamine. |