http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109929788-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-19 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-205 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2018-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109929788-B |
| titleOfInvention | Bacterial strain with ccdB negative screening effect and construction method thereof |
| abstract | The invention discloses a strain with ccdB negative screening effect, which is obtained by removing ccdA genes and TetR genes in F factors And (3) strain. The invention also discloses a method for constructing the strain with ccdB negative screening effect, which comprises the following steps: (1) by using Red/ET homologous recombination technology Replacing the ccdA and TetR genes on the F factor in the strain with an FRT-Kan cassette-FRT DNA sequence to obtain the F factor after gene editing; (2) providing a plasmid for expressing FLP recombinase, and deleting an exogenous sequence in the middle of two FRT sites on the F factor after gene editing through the FLP recombinase mediated recombination reaction expressed by the plasmid to obtain the strain with ccdB negative screening effect. The strain constructed by the invention has ccdB negative screening effect, can be applied to the construction of vectors based on the technologies such as Gateway and Golden Gate, and has good stable replication capacity on complex fragments such as repetitive sequences. |
| priorityDate | 2018-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.