http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109929788-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c53918f93b9d7419bc595f65788eb110 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-19 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-205 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2018-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4a70fed8ab98ac5fdd01db109adf8dac http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_06feb3255ed15d9f8e9d9a59c64113db http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_41a85764387986aa0e6a39391ac7dc8d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eeb16fb098d4bf1ac6d1c1097053497a |
| publicationDate | 2019-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109929788-A |
| titleOfInvention | A kind of bacterial strain and its construction method for bearing sieve effect with ccdB |
| abstract | The invention discloses a kind of bacterial strain for bearing sieve effect with ccdB, the bacterial strain is to eliminate ccdA gene and TetR gene in the F factor Bacterial strain.The invention also discloses the strain construction methods that sieve effect is born with ccdB, comprising the following steps: (1) utilizes Red/ET homologous recombination technique will CcdA and TetR gene replacement in bacterial strain in the F factor is FRT-Kan cassette-FRT DNA sequence dna, the F factor after obtaining gene editingļ¼(2) plasmid of expression FLP recombinase is provided, by the FLP recombinase-mediated recombining reaction of the plasmid expression, the exogenous array in the F factor after deleting the gene editing among two sites FRT is to get the bacterial strain with the negative sieve effect of ccdB.The bacterial strain that the present invention constructs has the negative sieve effect of ccdB, can be applied to the vector construction based on technologies such as Gateway and Golden Gate, which has good stable replication capacity to the complicated segment such as repetitive sequence. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110423771-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110423771-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111808884-A |
| priorityDate | 2018-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.