| abstract |
The invention discloses a primer for rapid detection of koi herpes virus and a method thereof; it aims to provide a primer and a method for rapid detection of koi herpes virus with strong specificity, high sensitivity and good repeatability; its technical scheme includes a primer core The primers whose nucleotide sequences are shown in SEQ ID NO: 1-SEQ ID NO: 3; the detection method is: 1) extracting viral genomic DNA from clinical samples; 2) using the extracted sample DNA as a template, using claim 1 Described primer set, claim 2 probe preparation RPA reaction system, adopts TwistAmp exo kit to enter RPA amplification reaction; 3) the reaction tube after RPA amplification is placed in fluorescence detector, real-time monitoring fluorescence signal, if If there is an obvious fluorescence signal curve, it means that the sample contains KHV, if there is no fluorescence signal curve, it means that the sample does not contain KHV; the invention belongs to the field of animal pathogen detection. |