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filingDate 2019-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6b706a702de73cbc72f755051a6928a6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2c4c90d63d3f7f28216d51b0a175e0ff
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publicationDate 2019-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-109913564-A
titleOfInvention A primer-probe composition, kit and method for detecting Chlamydia pneumoniae
abstract The present invention relates to a primer-probe composition for detecting Chlamydia pneumoniae, which comprises primers with sequences shown in SEQ ID No. 1 and SEQ ID No. 2 for amplifying target DNA fragments and primers as shown in SEQ ID No. The probe of the sequence shown in 3 also includes the primers of the sequence shown in SEQ ID No. 4 and SEQ ID No. 5 and the probe of the sequence of SEQ ID No. 6 for amplifying the internal standard DNA fragment. The present invention also relates to a kit containing the above-mentioned primer-probe composition and a method for detecting Chlamydia pneumoniae using the kit. In the present invention, primers and probes are designed according to the gene conserved region of Chlamydia pneumoniae (Cpn), and the Cpn gene is detected by real-time fluorescence quantitative PCR technology. The present invention performs real-time fluorescence quantitative PCR detection, greatly improves the sensitivity and specificity of detection, reduces detection time, and brings great help to the research of Cpn infectious diseases and the accurate clinical diagnosis of Cpn infectious diseases.
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priorityDate 2019-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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