http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109913517-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_aaadcd534c1edeab62f85ec7991cb759 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-14 |
| filingDate | 2019-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ec72a9296a08771120b8d22bfb628413 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2fd5a72c6a36654105c456fc11fc4796 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dba90957fcad3b3483af432b7aa55c3c |
| publicationDate | 2019-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109913517-A |
| titleOfInvention | Preparation and purification method of different region oligosaccharides of heparan sulfate/heparin |
| abstract | The invention relates to a preparation and purification method of oligosaccharides of different regions of heparan sulfate/heparin (HS/Hp). The method uses commercially available heparin as a raw material, utilizes heparinase I to specifically enzymatically hydrolyze the high thiobiose containing 3 sulfite groups, precisely controls the enzymatic hydrolysis reaction conditions to achieve partial enzymatic hydrolysis, and obtains a high thiobiose containing high thiobiose. The sulfated region oligosaccharides also enriched the low sulfated oligosaccharide fragments containing NS and NAc structures in HS/Hp, thereby preparing a series of low sulfated region oligosaccharides with different structures. A series of low sulfated HS/Hp oligosaccharides were separated and purified by gel chromatography and ion exchange high performance liquid chromatography. The method for preparing oligosaccharides in different regions of HS/Hp established by the invention is fast, simple and low in cost, and provides an important sugar library sample for the structure and function research of HS/Hp, and also provides an important source for the development of new heparin drugs. Precursor. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111763702-A |
| priorityDate | 2019-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 32.