http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109913413-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0783 |
| filingDate | 2019-03-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109913413-B |
| titleOfInvention | PD-1 antibody loaded T cell in-vitro culture method, cell preparation and application thereof |
| abstract | The embodiment of the invention discloses a PD-1 antibody loaded T cell in-vitro culture method, a cell preparation and application thereof, relating to the technical field of immunotherapy, in particular to the culture method comprising the following steps: it comprises mixing peripheral blood mononuclear cells with an A-antibody culture medium for primary culture; then, mixing the product obtained by the first culture with a B-type culture medium for the second culture; finally, the product obtained after the second culture is mixed with a K-type culture medium. The culture method can induce lymphocytes separated from whole blood to proliferate and differentiate towards CD8+ T cells in the culture process, the separated T cells can achieve more than 40 times of amplification within 18 days, and the proportion of CD8+ T cells is more than 80%. |
| priorityDate | 2019-03-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 156.