http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109912721-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 |
filingDate | 2019-04-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-03-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-03-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-109912721-B |
titleOfInvention | Method for creating insect-resistant fusion gene and application thereof |
abstract | The invention discloses a method for creating an insect-resistant fusion gene and application thereof. The invention provides a method for creating an insect-resistant fusion protein, which comprises the following steps: connecting the endo _ N structural domain and the endo _ M structural domain of the insect-resistant protein A at the amino acid with the delta _ endoxin _ C structural domain of the insect-resistant protein B at the carboxyl terminal to obtain the insect-resistant fusion protein. The insect-resistant fusion gene (cry1A.172, cry1F.148 or cry1A.207) created by the method is introduced into a maize inbred line B73, and the obtained transgenic maize has strong toxicity to lepidoptera insects (such as corn borer) and does not affect the original agronomic traits of a receptor plant. The invention has important significance for creating the Bt gene with insecticidal effect on target pests, increasing the diversity of Bt proteins and widening the binding sites of the Bt proteins for the target pests. |
priorityDate | 2019-04-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 607.