| abstract |
The invention discloses a kind of aptamer fluorescent optical sensors and its construction method for detecting myoglobins, belong to technical field of analysis and detection.The construction method, comprising: the myoglobins aptamer for being modified with quenching group and the aptamer complementary strand for being modified with fluorophor are added in buffer system and hybridize, the aptamer fluorescent optical sensor is made.When addition myoglobins, aptamer is separated with complementary short-chain, in conjunction with myoglobins, be connected with fluorophor complementary short-chain disengaging be connected with the aptamer of quenching group after, the fluorescence of fluorophor is restored, the intensity that its fluorescence restores is directly proportional to the concentration of myoglobins, thus can measure the concentration of myoglobins.The present invention detects the accurate detection, it can be achieved that myoglobins to myoglobins using standby aptamer fluorescent optical sensor cheap and easy to manufacture.This method design is simple, at low cost, detecting step is few, easy to operate, high sensitivity, and selectivity is good. |